Journal: PLOS One
Article Title: Proteomic profiling of a Sporobolomyces yeast reveals global responses to UV-B–induced oxidative stress
doi: 10.1371/journal.pone.0333568
Figure Lengend Snippet: The figure displays the DPPH free radical quenching activity of extracts of yeast isolates LEV-2 (A) , LEV-9 (B) and LEV-13 (C) . Yeast cultures were irradiated for time periods ranging from 5 minutes (sample T.5) to 24 hours (sample T.24), using dual Philips Ultraviolet-B TL 20W/12RS lamps, and yeast extracts were obtained by cell lysis and removal of insoluble material. The controls were half-strength YPD medium (sample M) , non-irradiated yeast cultures (sample T.0), and non-irradiated yeast cultures grown for 24 hours (sample T.24.C). Error bars indicate 1 standard deviation of the mean, calculated from three experiments; DPPH quenching values of three technical triplicates of each experiment were averaged.
Article Snippet: Yeast cultures were placed in open Petri dishes and irradiated under dual Philips TL 20W/12RS UV-B lamps, which provided a UV-B output of 4 J/m2/s and UV-A output of 1.75 J/m2/s, at a distance of 10 cm from the light source.
Techniques: Activity Assay, Irradiation, Lysis, Standard Deviation